Optimization of a Whole-Blood Gamma Interferon Assay for Detection of Mycobacterium bovis-Infected Cattle

Antigens of Mycobacterium bovis elicit a cell-mediated immune response upon intradermal injection in cattle. In vitro, such antigens stimulate the production of gamma interferon (IFN-γ) by bovine T cells in whole-blood culture (IFN-γ assay). We have analyzed various parameters of the in vitro IFN-γ...

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Auteurs principaux: Schiller, Irene, Waters, W. Ray, Vordermeier, H. Martin, Nonnecke, Brian, Welsh, Michael, Keck, Nicolas, Whelan, Adam, Sigafoose, Teresa, Stamm, Christoph, Palmer, Mitchell, Thacker, Tyler, Hardegger, Roland, Marg-Haufe, Beatrice, Raeber, Alex, Oesch, Bruno
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2009
Sujets:
Veterinary Immunology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725547/
https://ncbi.nlm.nih.gov/pubmed/19571108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00150-09
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