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Assessment of Bias Associated with Incomplete Extraction of Microbial DNA from Soil

DNA extraction bias is a frequently cited but poorly understood limitation of molecular characterizations of environmental microbial communities. To assess the bias of a commonly used soil DNA extraction kit, we varied the cell lysis protocol and conducted multiple extractions on subsamples of clay,...

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Detalhes bibliográficos
Main Authors: Feinstein, Larry M., Sul, Woo Jun, Blackwood, Christopher B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725469/
https://ncbi.nlm.nih.gov/pubmed/19561189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00120-09
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