Plasmid-Based System for High-Level Gene Expression and Antisense Gene Knockdown in Bartonella henselae

Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a β-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of...

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Autors principals: Gillaspie, Devin, Perkins, Izabella, Larsen, Kellie, McCord, Amy, Pangonis, Scott, Sweger, Debra, Seleem, Mohamed N., Sriranganathan, Nammalwar, Anderson, Burt E.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2009
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Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725459/
https://ncbi.nlm.nih.gov/pubmed/19542333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00949-09
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