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Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates
BACKGROUND: Conditional knockout mice are a useful tool to study the function of gene products in a tissue-specific or inducible manner. Classical approaches to generate targeting vectors for conditional alleles are often limited by the availability of suitable restriction sites. Furthermore, plasmi...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2724507/ https://ncbi.nlm.nih.gov/pubmed/19638212 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-69 |
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