Fractionation of Pneumocystis carinii antigens used in an enzyme-linked immunosorbent assay for antibodies and in the production of antiserum for detecting Pneumocystis carinii antigenemia.

Cyst-rich suspensions of Pneumocystis carinii were obtained by differential and gradient centrifugation from heavily infected rat lungs. After preparation of an aqueous-soluble extract of the cyst-rich material, the insoluble residue was extracted with 8 M urea. Small amounts of infected human lung...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Maddison, S E, Hayes, G V, Ivey, M H, Tsang, V C, Slemenda, S B, Norman, L G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1982
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC272248/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6980894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.15.6.1029-1035.1982
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