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One-step purification of a recombinant protein from a whole cell extract by reversed-phase high-performance liquid chromatography
We have developed a one-step facile, flexible and readily scalable purification method for a recombinant protein, TM 1–99 (113 amino acid residues; 12,837 Da) based on reversed-phase high-performance liquid chromatography (RP-HPLC) from an E. coli cell lysate. Following cell lysis, the cell contents...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2722115/ https://ncbi.nlm.nih.gov/pubmed/16945380 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2006.08.042 |
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