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Rapid micromethod for preparation of enzyme-antibody conjugates.

A rapid method is described for labeling antibody with alkaline phosphatase by one-step glutaraldehyde linkage. The method involves the centrifugation of a small volume of an enzyme and antibody mixture through a minicolumn packed with hydrated Sephadex. This procedure rapidly removes ammonium sulfa...

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Detalles Bibliográficos
Main Authors: Parkinson, A J, Scott, E N, Muchmore, H G
Formato: Artigo
Idioma:Inglês
Publicado: 1982
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC272177/
https://ncbi.nlm.nih.gov/pubmed/6802872
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