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Isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma

AIM: To explore the method of isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma cell line PANC-1. METHODS: The PANC-1 cells were cultured in Dulbecco modified eagle medium F12 (1:1 volume) (DMEM-F12) supplemented with 20% fetal bovine serum (FBS). Subpopulation cel...

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Hlavní autoři: Huang, Peng, Wang, Chun-You, Gou, Shan-Miao, Wu, He-Shui, Liu, Tao, Xiong, Jiang-Xin
Médium: Artigo
Jazyk:Inglês
Vydáno: The WJG Press and Baishideng 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2721450/
https://ncbi.nlm.nih.gov/pubmed/18609717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.14.3903
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