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Crystallization and preliminary X-ray analysis of eukaryotic initiation factor 4E from Pisum sativum

Crystals of an N-terminally truncated 20 kDa fragment of Pisum sativum eIF4E (ΔN-eIF4E) were grown by vapour diffusion. X-ray data were recorded to a resolution of 2.2 Å from a single crystal in-house. Indexing was consistent with primitive monoclinic symmetry and solvent-content estimations suggest...

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Detalhes bibliográficos
Main Authors: Ashby, Jamie A., Stevenson, Clare E. M., Maule, Andrew J., Lawson, David M.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2720347/
https://ncbi.nlm.nih.gov/pubmed/19652353
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109027389
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