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Expression and purification of cysteine introduced recombinant saporin
Saporin, a ribosome inactivating protein is widely used for immunotoxin construction. Here we describe a mutation of saporin (sap)-3 DNA by introducing a cysteine residue, followed by protein expression and purification by ion exchange chromatography. The purified Cys255sap-3, sap-3 isomer and comme...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2719709/ https://ncbi.nlm.nih.gov/pubmed/18164211 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.11.005 |
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