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Dynamics and folding of single two-stranded coiled-coil peptides studied by fluorescent energy transfer confocal microscopy

We report single-molecule measurements on the folding and unfolding conformational equilibrium distributions and dynamics of a disulfide crosslinked version of the two-stranded coiled coil from GCN4. The peptide has a fluorescent donor and acceptor at the N termini of its two chains and a Cys disulf...

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Published in:Proc Natl Acad Sci U S A
Main Authors: Talaga, David S., Lau, Wai Leung, Roder, Heinrich, Tang, Jianyong, Jia, Yiwei, DeGrado, William F., Hochstrasser, Robin M.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2000
Subjects:
Physical Sciences
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27171/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11087856/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.24.13021
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