A capsid gene-based real-time reverse transcription polymerase chain reaction assay for the detection of marine vesiviruses in the Caliciviridae

A real-time reverse transcription polymerase chain reaction (rtRT-PCR) assay was developed for the identification of marine vesiviruses. The primers were designed to target a 176-nucleotide fragment within a highly conserved region of the San Miguel sea lion viruses (SMSVs) capsid gene. The assay de...

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Main Authors: McClenahan, Shasta D., Bok, Karin, Neill, John D., Smith, Alvin W., Rhodes, Crystal R., Sosnovtsev, Stanislav V., Green, Kim Y., Romero, Carlos H.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier B.V. Published by Elsevier B.V. 2009
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2716420/
https://ncbi.nlm.nih.gov/pubmed/19410604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.04.026
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