Construction and functional analyses of a comprehensive σ(54) site-directed mutant library using alanine–cysteine mutagenesis

The σ(54) factor associates with core RNA polymerase (RNAP) to form a holoenzyme that is unable to initiate transcription unless acted on by an activator protein. σ(54) is closely involved in many steps of activator-dependent transcription, such as core RNAP binding, promoter recognition, activator...

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Main Authors: Xiao, Yan, Wigneshweraraj, Siva R., Weinzierl, Robert, Wang, Yi-Ping, Buck, Martin
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2009
Assuntos:
Gene Regulation, Chromatin and Epigenetics
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2715252/
https://ncbi.nlm.nih.gov/pubmed/19474350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp419
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