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Selecting Folded Proteins from a Library of Secondary Structural Elements
A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands and loops) are assembled semi-randomly into sequences comprised of as many as 800 amino acid residues. A library of novel polypeptides...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2714538/ https://ncbi.nlm.nih.gov/pubmed/18067292 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja074405w |
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