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Frequency-pulsed electron capture gas-liquid chromatographic analysis of metabolites produced by Clostridium difficile in broth enriched with amino acids.

Clostridium difficile strain CDC A-567 was cultured in Trypticase (BBL Microbiology Systems)-yeast-salt broth supplemented with 0.2% L-leucine, L-norleucine, L-isoleucine, L-tyrosine, or L-tryptophan. Four extractions were done on the spent medium, three at pH 2 and one at pH 10, using CHCL3 or ethe...

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Main Authors: Brooks, J B, Nunez-Montiel, O L, Wycoff, B J, Moss, C W
格式: Artigo
語言:Inglês
出版: 1984
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC271368/
https://ncbi.nlm.nih.gov/pubmed/6490835
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