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S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI

In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-li...

詳細記述

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書誌詳細
主要な著者: Peakman, Luke J., Szczelkun, Mark D.
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 2009
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709564/
https://ncbi.nlm.nih.gov/pubmed/19401438
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp267
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