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Real-Time PCR-Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes

A real-time reverse-transcription PCR was developed to detect and pathotype Newcastle disease viruses (NDV) in clinical samples. Degenerate oligonucleotide primers and TaqMan probes with nonfluorescent minor groove binder (MGB) quencher amplified and hybridized to a region in the fusion protein (F)...

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Bibliografske podrobnosti
Main Authors: Farkas, T., Székely, É., Belák, S., Kiss, I.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2708511/
https://ncbi.nlm.nih.gov/pubmed/19439542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01652-08
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