Real-Time PCR-Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes

A real-time reverse-transcription PCR was developed to detect and pathotype Newcastle disease viruses (NDV) in clinical samples. Degenerate oligonucleotide primers and TaqMan probes with nonfluorescent minor groove binder (MGB) quencher amplified and hybridized to a region in the fusion protein (F)...

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Bibliografiska uppgifter
Huvudupphovsmän: Farkas, T., Székely, É., Belák, S., Kiss, I.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology (ASM) 2009
Ämnen:
Virology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2708511/
https://ncbi.nlm.nih.gov/pubmed/19439542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01652-08
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