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Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein–protein interactions using global analysis

Förster resonance energy transfer (FRET) detected via fluorescence lifetime imaging microscopy (FLIM) and global analysis provide a way in which protein–protein interactions may be spatially localized and quantified within biological cells. The FRET efficiency and proportion of interacting molecules...

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Autori principali: Barber, P.R., Ameer-Beg, S.M., Gilbey, J., Carlin, L.M., Keppler, M., Ng, T.C., Vojnovic, B.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Royal Society 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2706460/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsif.2008.0451.focus
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