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Storage conditions of blood samples and primer selection affect the yield of cDNA polymerase chain reaction products of hepatitis C virus.

We have noticed that suboptimal specimen processing and storage conditions may cause false-negative results in the detection of hepatitis C virus (HCV) RNA in plasma or serum. To establish the influence of specimen handling in a serological laboratory on the rate of detection of HCV RNA by the cDNA...

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Publicat a:J Clin Microbiol
Autors principals: Cuypers, H T, Bresters, D, Winkel, I N, Reesink, H W, Weiner, A J, Houghton, M, van der Poel, C L, Lelie, P N
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270636/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1333489/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.12.3220-3224.1992
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