The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples.

Direct polymerase chain reaction (PCR)-based detection with fecal specimens is hampered by inhibitory compounds, such as bilirubin and bile salts. These fecal compounds showed significant inhibition of PCR at low concentrations (10 to 50 micrograms/ml). For direct PCR analysis, fecal samples must be...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Main Authors: Widjojoatmodjo, M N, Fluit, A C, Torensma, R, Verdonk, G P, Verhoef, J
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270624/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1452702/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.12.3195-3199.1992
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