Construction and characterization of a bovine BAC library with four genome-equivalent coverage

A bovine artificial chromosome (BAC) library of 105 984 clones has been constructed in the vector pBeloBAC11 and organized in 3-dimension pools and high density membranes for screening by PCR and hybridization. The average insert size, determined after analysis of 388 clones, was estimated at 120 kb...

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Main Authors: Eggen, André, Gautier, Mathieu, Billaut, Alain, Petit, Élisabeth, Hayes, Hélène, Laurent, Pascal, Urban, Catherine, Pfister-Genskow, Martha, Eilertsen, Ken, Bishop, Michael D
格式: Artigo
語言:Inglês
出版: BioMed Central 2001
主題:
Research
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2705404/
https://ncbi.nlm.nih.gov/pubmed/11712974
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1297-9686-33-5-543
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