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Detection of Babesia bigemina-infected carriers by polymerase chain reaction amplification.

A SpeI-AvaI fragment (0.3 kbp) from pBbi16 (a pBR322 derivative containing a 6.3-kbp Babesia bigemina DNA insert) was subcloned into the pBluescript phagemid vector and was sequenced by the dideoxy-mediated chain termination method. Two sets of primers were designed for the polymerase chain reaction...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Figueroa, J V, Chieves, L P, Johnson, G S, Buening, G M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270481/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1400956/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.10.2576-2582.1992
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