Detection of rodent coronaviruses in tissues and cell cultures by using polymerase chain reaction.

A polymerase chain reaction (PCR) method was developed for the detection of rodent coronaviruses in biological material by using reverse transcriptase and two primers which flanked an M gene sequence of 375 bp. PCR detected all of 11 different strains of mouse hepatitis virus (MHV) as well as rat si...

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Vydáno v:	J Clin Microbiol
Hlavní autoři:	Homberger, F R, Smith, A L, Barthold, S W
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1991
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270434/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1661745/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.12.2789-2793.1991
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