Chromatographic purification and characterization of antigens A and D from Mycobacterium paratuberculosis and their use in enzyme-linked immunosorbent assays for diagnosis of paratuberculosis in sheep.

The protein antigens A and D were purified from culture filtrates and sonic extracts of laboratory strains of Mycobacterium paratuberculosis by salt precipitation and chromatography. The characterization of antigen A is shown here, and both antigens were evaluated along with lipoarabinomannan antige...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Sugden, E A, Brooks, B W, Young, N M, Watson, D C, Nielsen, K H, Corner, A H, Turcotte, C, Michaelides, A, Stewart, R B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270180/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1761688/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.8.1659-1664.1991
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