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Detection of the Lyme disease bacterium, Borrelia burgdorferi, by using the polymerase chain reaction and a nonradioisotopic gene probe.

A 419-bp region of the flagellin gene sequence of Borrelia burgdorferi was used as a target for the polymerase chain reaction. With a nonradioactively labeled gene-specific probe, sensitivity to as few as 1 to 10 spirochetes was observed. The targeted gene fragment was conserved in the American and...

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Vydáno v:J Clin Microbiol
Hlavní autoři: Wise, D J, Weaver, T L
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270147/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1885749/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.7.1523-1526.1991
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