Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis.

A set of synthetic oligonucleotide primers was designed for use in a polymerase chain reaction protocol to specifically detect the B subunit genes in vtx2ha and vtx2hb, which code for the production of the VT2 (Shiga-like toxin II) variant cytotoxins VT2v-a and VT2v-b, respectively. An additional se...

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Main Authors: Tyler, S D, Johnson, W M, Lior, H, Wang, G, Rozee, K R
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC270112/
https://ncbi.nlm.nih.gov/pubmed/1679436
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