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Two-Step Versus One-Step RNA-to-C(T)™ 2-Step and One-Step RNA-to-C(T)™ 1-Step: Validity, Sensitivity, and Efficiency
Quantitative RT-PCR can be carried out as a one- or a two-step reaction. However, the choice of method raises controversy from the perspective of the researcher and manufacturer, because of advantages and disadvantages with both systems. We therefore hypothesize that running the RNA-to-C(T)™ 2-Step...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Association of Biomolecular Resource Facilities
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2700466/ https://ncbi.nlm.nih.gov/pubmed/19568456 |
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