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Two-Step Versus One-Step RNA-to-C(T)™ 2-Step and One-Step RNA-to-C(T)™ 1-Step: Validity, Sensitivity, and Efficiency

Quantitative RT-PCR can be carried out as a one- or a two-step reaction. However, the choice of method raises controversy from the perspective of the researcher and manufacturer, because of advantages and disadvantages with both systems. We therefore hypothesize that running the RNA-to-C(T)™ 2-Step...

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Détails bibliographiques
Auteurs principaux: Al-Shanti, Nasser, Saini, Amarjit, Stewart, Claire E.
Format: Artigo
Langue:Inglês
Publié: Association of Biomolecular Resource Facilities 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2700466/
https://ncbi.nlm.nih.gov/pubmed/19568456
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