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Two-Step Versus One-Step RNA-to-C(T)™ 2-Step and One-Step RNA-to-C(T)™ 1-Step: Validity, Sensitivity, and Efficiency

Quantitative RT-PCR can be carried out as a one- or a two-step reaction. However, the choice of method raises controversy from the perspective of the researcher and manufacturer, because of advantages and disadvantages with both systems. We therefore hypothesize that running the RNA-to-C(T)™ 2-Step...

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書目詳細資料
Main Authors: Al-Shanti, Nasser, Saini, Amarjit, Stewart, Claire E.
格式: Artigo
語言:Inglês
出版: Association of Biomolecular Resource Facilities 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2700466/
https://ncbi.nlm.nih.gov/pubmed/19568456
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