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Expression and Purification of Isotopically Labeled Peptide Inhibitors and Substrates of cAMP-Dependant Protein Kinase A for NMR Analysis

Extensive x-ray crystallographic studies carried out on the catalytic-subunit of protein kinase A (PKAc) enabled the atomic characterization of inhibitor and/or substrate peptide analogs trapped at its active site. Yet, the structural and dynamic transitions of these peptides from the free to the bo...

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Main Authors: Masterson, Larry R., Bortone, Nadia, Yu, Tao, Ha, Kim N., Gaffarogullari, Ece C., Nguyen, Oanh, Veglia, Gianluigi
Format: Artigo
Jezik:Inglês
Izdano: 2008
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2699774/
https://ncbi.nlm.nih.gov/pubmed/19027069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.10.016
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