Characterization of the Escherichia coli ClpY (HslU) Substrate Recognition Site in the ClpYQ (HslUV) Protease Using the Yeast Two-Hybrid System

In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease in which ClpQ is the peptidase subunit and ClpY is the ATPase and the substrate-binding subunit. The ATP-dependent proteolysis is mediated by substrate recognition in the ClpYQ complex. ClpY has three domains, N, I, and C,...

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Bibliographic Details
Main Authors: Lien, Hsiang-Yun, Shy, Ru-Shan, Peng, Sheng-Shiang, Wu, Yuei-Long, Weng, Yu-Ting, Chen, Hsuan-He, Su, Pin-Chih, Ng, Wei-Fu, Chen, Yu-Chun, Chang, Pei-Yi, Wu, Whei-Fen
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Enzymes and Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2698502/
https://ncbi.nlm.nih.gov/pubmed/19395483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00089-09
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