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Comparison of biotinylated DNA and RNA probes for rapid detection of varicella-zoster virus genome by in situ hybridization.

We describe a general method for the production of nonisotopic DNA and RNA probes for the detection of the varicella-zoster virus (VZV) genome by in situ hybridization. VZV DNA was extracted from purified viral nucleocapsids, cleaved with restriction enzyme (RE) BamHI, and cloned into plasmid pBR322...

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Vydáno v:J Clin Microbiol
Hlavní autoři: Forghani, B, Yu, G J, Hurst, J W
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269823/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1645371/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.3.583-591.1991
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