Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus

A bacterial artificial chromosome (BAC) library was constructed by cloning HindIII-digested high molecular weight DNA from a gynogenetic channel catfish, Ictalurus punctatus, into the vector pBeloBAC11. Approximately 53 500 clones were arrayed in 384-well plates and stored at -80°C (CCBL1), while cl...

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Autors principals: Quiniou, Sylvie MA, Katagiri, Takayuki, Miller, Norman W, Wilson, Melanie, Wolters, William R, Waldbieser, Geoffrey C
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2003
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2698005/
https://ncbi.nlm.nih.gov/pubmed/14604514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1297-9686-35-7-673
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