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Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens.

The rotavirus gene segment coding for the major outer capsid glycoprotein vp7 was amplified directly from stool specimens by the polymerase chain reaction (PCR). Double-stranded RNA extracted from stool samples was used as the template for reverse transcription, which was followed immediately and in...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Autores principales: Gouvea, V, Glass, R I, Woods, P, Taniguchi, K, Clark, H F, Forrester, B, Fang, Z Y
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 1990
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269590/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2155916/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.2.276-282.1990
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