Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens.
The rotavirus gene segment coding for the major outer capsid glycoprotein vp7 was amplified directly from stool specimens by the polymerase chain reaction (PCR). Double-stranded RNA extracted from stool samples was used as the template for reverse transcription, which was followed immediately and in...
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| Published in: | J Clin Microbiol |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1990
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269590/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2155916/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.2.276-282.1990 |
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