Mung Bean nuclease mapping of RNAs 3' end

A method is described that allows an accurate mapping of 3' ends of RNAs. In this method a labeled DNA probe, containing the presumed 3' end of the RNA under analysis is allowed to anneals to the RNA itself. Mung-bean nuclease is then used to digest single strands of both RNA and DNA. Elec...

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Bibliographic Details
Main Authors: Bellavia, Daniele, Sisino, Giorgia, Papadopoulos, Giorgio L, Forte, Giusi I, Barbieri, Rainer
Format: Artigo
Language:Inglês
Published: BioMed Central 2009
Subjects:
Short Report
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2695431/
https://ncbi.nlm.nih.gov/pubmed/19460154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1742-4933-6-6
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