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Functional and shunt states of bacteriorhodopsin resolved by 250 GHz dynamic nuclear polarization–enhanced solid-state NMR

Observation and structural studies of reaction intermediates of proteins are challenging because of the mixtures of states usually present at low concentrations. Here, we use a 250 GHz gyrotron (cyclotron resonance maser) and cryogenic temperatures to perform high-frequency dynamic nuclear polarizat...

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Détails bibliographiques
Auteurs principaux: Bajaj, Vikram S., Mak-Jurkauskas, Melody L., Belenky, Marina, Herzfeld, Judith, Griffin, Robert G.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2695048/
https://ncbi.nlm.nih.gov/pubmed/19474298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0900908106
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