Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization.

Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Ticehurst, J R, Feinstone, S M, Chestnut, T, Tassopoulos, N C, Popper, H, Purcell, R H
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1987
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269349/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822759/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.25.10.1822-1829.1987
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