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Automated screening for mutants affecting dopaminergic neuron specification in C. elegans

We describe an automated method to isolate mutant C. elegans animals, which fail to appropriately execute a cellular differentiation program that we monitor with gfp-based reporter gene technology. A fluorescence activated sorting mechanism implemented in the COPAS Biosort machine is able to isolate...

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Autors principals: Doitsidou, Maria, Flames, Nuria, Lee, Albert C., Boyanov, Alexander, Hobert, Oliver
Format: Artigo
Idioma:Inglês
Publicat: 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2693092/
https://ncbi.nlm.nih.gov/pubmed/18758453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1250
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