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Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
We developed a simple, direct and cost-effective approach to search for the most likely target genes of a known microRNA (miRNA) in vitro. We term this method ‘labeled miRNA pull-down (LAMP)’ assay system. Briefly, the pre-miRNA is labeled with digoxigenin (DIG), mixed with cell extracts and immunop...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2691847/ https://ncbi.nlm.nih.gov/pubmed/19420057 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp274 |
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