Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

BACKGROUND: Despite the powerful impact in recent years of gene expression markers like the green fluorescent protein (GFP) to link the expression of recombinant protein for selection of high producers, there is a strong incentive to develop rapid and efficient methods for isolating mammalian cell c...

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Detalhes bibliográficos
Main Authors: Caron, Antoine W, Nicolas, Claire, Gaillet, Bruno, Ba, Ismaïla, Pinard, Maxime, Garnier, Alain, Massie, Bernard, Gilbert, Rénald
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2689207/
https://ncbi.nlm.nih.gov/pubmed/19432976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-42
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