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Crystallization of Mycobacterium smegmatis methionyl-tRNA synthetase in the presence of methionine and adenosine

Methionyl-tRNA synthetase (MetRS) from Mycobacterium smegmatis was recombinantly expressed in Escherichia coli and purified using Ni(2+)-affinity and size-exclusion chromatography. Crystals formed readily in the presence of the ligands methionine and adenosine. These two ligands are components of an...

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Bibliografiske detaljer
Main Authors: Ingvarsson, Henrik, Jones, T. Alwyn, Unge, Torsten
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2688425/
https://ncbi.nlm.nih.gov/pubmed/19478446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109016704
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