Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase

Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase (SacKdgA) displays optimal activity at 95 °C and is studied as a model enzyme for aldol condensation reactions. For application of SacKdgA at lower temperatures, a library of randomly generated mutants was screened for improved synthesis of...

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Autors principals: Wolterink-van Loo, Suzanne, Siemerink, Marco A. J., Perrakis, Georgios, Kaper, Thijs, Kengen, Servé W.M., van der Oost, John
Format: Artigo
Idioma:Inglês
Publicat: Heron Publishing 2009
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Research Articles
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2686391/
https://ncbi.nlm.nih.gov/pubmed/19478916
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