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Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin.

High titers of Verotoxin (VT) were released from cell pellets of VT-producing Escherichia coli (VTEC; corresponding to E. coli strains producing "high" levels of Shiga-like toxin) after incubation in polymyxin B (0.1 mg/ml) for 30 min at 37 degrees C. Maximal titers of polymyxin-releasable VT occurr...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Karmali, M A, Petric, M, Lim, C, Cheung, R, Arbus, G S
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC268478/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3908473/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.22.4.614-619.1985
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