Rapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis.

We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 micrograms of chromosomal DNA. In a 1-day prot...

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Vydáno v:	J Clin Microbiol
Hlavní autoři:	Le Febvre, R B, Foley, J W, Thiermann, A B
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1985
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC268476/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001135/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.22.4.606-608.1985
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