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Enhancement of the Ca(2+)-triggering steps of native membrane fusion via thiol-reactivity
Ca(2+)-triggered membrane fusion is the defining step of exocytosis. Isolated urchin cortical vesicles (CV) provide a stage-specific preparation to study the mechanisms by which Ca(2+) triggers the merger of two apposed native membranes. Thiol-reactive reagents that alkylate free sulfhydryl groups o...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Springer-Verlag
2008
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2682352/ https://ncbi.nlm.nih.gov/pubmed/19568790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12154-008-0013-3 |
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