Pyrosequencing of the Chaperonin-60 Universal Target as a Tool for Determining Microbial Community Composition

We compared dideoxy sequencing of cloned chaperonin-60 universal target (cpn60 UT) amplicons to pyrosequencing of amplicons derived from vaginal microbial communities. In samples pooled from a number of individuals, the pyrosequencing method produced a data set that included virtually all of the seq...

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Bibliographic Details
Main Authors: Schellenberg, John, Links, Matthew G., Hill, Janet E., Dumonceaux, Tim J., Peters, Geoffrey A., Tyler, Shaun, Ball, T. Blake, Severini, Alberto, Plummer, Francis A.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2681704/
https://ncbi.nlm.nih.gov/pubmed/19270139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01640-08
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