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Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel i...

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Bibliografiske detaljer
Main Authors: Ferrari, B. C., Gillings, M. R.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2681634/
https://ncbi.nlm.nih.gov/pubmed/19304839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02407-08
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