Identification of a double-stranded RNA virus by using polymerase chain reaction and magnetic separation of the synthesized DNA segments.

A double-nested polymerase chain reaction assay (PCR), followed by magnetic separation of the PCR-synthesized DNA segments, was developed to detect a double-stranded RNA virus, infectious pancreatic necrosis virus from salmonid fish. Viral RNA was extracted from cell cultures and used for cDNA synth...

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Библиографические подробности
Главные авторы: Rimstad, E, Hornes, E, Olsvik, O, Hyllseth, B
Формат: Artigo
Язык:Inglês
Опубликовано: 1990
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC268161/
https://ncbi.nlm.nih.gov/pubmed/2229352
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