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Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans

In order to determine how signaling pathways differentially regulate gene expression, it is necessary to identify the interactions between transcription factors (TFs) and their cognate cis-regulatory DNA elements. Here, we have outlined a chromatin immunoprecipitation (ChIP) protocol for use in whol...

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Bibliografiska uppgifter
Huvudupphovsmän: Mukhopadhyay, Arnab, Deplancke, Bart, Walhout, Albertha J M, Tissenbaum, Heidi A
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2008
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2681100/
https://ncbi.nlm.nih.gov/pubmed/18388953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.38
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