Use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of Campylobacter jejuni and Campylobacter coli.

A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to detect either 5 ng of C. jejuni DNA or 10(5) CFU of bacteria. The SNAP could also detect DNA extract...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Olive, D M, Johny, M, Sethi, S K
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1990
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267989/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2380381/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.7.1565-1569.1990
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