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Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers.

A simple, sensitive, and specific polymerase chain reaction (PCR) protocol for the detection of human immunodeficiency virus type 1 (HIV-1) is described. We have improved all three PCR steps: sample preparation, DNA amplification, and detection of the amplified product. Some of the improvements have...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Principais autores: Albert, J, Fenyö, E M
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1990
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267988/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2380380/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.7.1560-1564.1990
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